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Structured Review

Promega padvantage e1711
Reagents and tools table
Padvantage E1711, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/padvantage+e1711/padvantage/pmc11790967-22-0-2
Average 90 stars, based on 1 article reviews
padvantage e1711 - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease"

Article Title: A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

Journal: The EMBO Journal

doi: 10.1038/s44318-024-00328-6

Reagents and tools table
Figure Legend Snippet: Reagents and tools table

Techniques Used: Recombinant, Transduction, CRISPR, Gene Knockout, Expressing, Concentration Assay, Immunofluorescence, Western Blot, Flow Cytometry, Sequencing, Red Blood Cell Lysis, Cell Recovery, Plasmid Preparation, SYBR Green Assay, Reverse Transcription, Software, Microscopy, Magnetic Beads, Transmission Assay

Related Articles

Recombinant:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Transduction:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

CRISPR:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Gene Knockout:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Expressing:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Concentration Assay:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Immunofluorescence:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Western Blot:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Flow Cytometry:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Sequencing:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Red Blood Cell Lysis:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Cell Recovery:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Plasmid Preparation:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

SYBR Green Assay:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Reverse Transcription:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Software:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Microscopy:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Magnetic Beads:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Transmission Assay:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Virus:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Multiplex Assay:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Saline:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Staining:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Incubation:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Imaging:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Control:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Retroviral:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).

Transfection:

Article Title: Dissecting the role of the tubulin code in mitosis
Article Snippet: pAdVantage , E1711, Promega.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-SWT, or FLAG-CIC-SCH1~5A, the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAGCIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1~5A-GFP, or MIGR1GFP control retroviral vector using FuGENE HD.

Article Title: Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins
Article Snippet: pAdVAntage , Promega , E1711.

Article Title: ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth
Article Snippet: To generate retroviruses expressing control, FLAG-CIC-S WT , or FLAG-CIC-S CH1∼5A , the HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-S WT -GFP, MIGR1-FLAG-CIC-S CH1∼5A -GFP, or MIGR1-GFP control retroviral vector using FuGENE HD.

Article Title: A functional genetic toolbox for human tissue-derived organoids
Article Snippet: We grew HEK293T cells in 10 cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 μg) with packaging vectors including pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according to the manufacturer’s protocol.



Article Title: Comparative haploid genetic screens reveal divergent pathways in the biogenesis and trafficking of glycophosphatidylinositol-anchored proteins
Article Snippet: Briefly, gene-trap retroviruses were produced by transfecting six T175 flasks of 293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, #E1711), pGAL and pCMV-VSVG using TurboFectin (Origene, #TF81001).



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Reagents and tools table

Journal: The EMBO Journal

Article Title: A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

doi: 10.1038/s44318-024-00328-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: pAdvantage , Promega , , E1711.

Techniques: Recombinant, Transduction, CRISPR, Gene Knockout, Expressing, Concentration Assay, Immunofluorescence, Western Blot, Flow Cytometry, Sequencing, Red Blood Cell Lysis, Cell Recovery, Plasmid Preparation, SYBR Green Assay, Reverse Transcription, Software, Microscopy, Magnetic Beads, Transmission Assay